Skip to main content
. 2022 Nov 25;8(47):eadc9346. doi: 10.1126/sciadv.adc9346

Fig. 1. KLF4 is highly expressed in the transitory effector subset of exhausted CD8 T cells with cytolytic effector function.

Fig. 1.

(A to G) scRNA-seq data from B16F10 melanoma–infiltrating CD8+ TILs (GSE116390) were analyzed. Pdcd1-expressing CD8+ TILs were filtered. (A) Clustering and uniform manifold approximation and projection (UMAP) visualization of Pdcd1-expressing CD8 T cells from B16F10 melanoma tumor (n = 2652 cells) on day 15. Colors denote transcriptional clusters, labeled with functional annotations. (B) UMAP visualization of transitory signature score within Pdcd1-expressing CD8+ TILs. (C) Violin plot of transitory signature score within clusters. Cluster 5 denotes transitory effector cells. (D to G) Violin plot of the expression level of (D) Tox, (E) Tbx21, (F) Tnf, and (G) Klf4 within clusters. Cluster 5 denotes transitory effector cells. (H to K) Five different CD8 T cell subpopulations were isolated from MC38 tumor–bearing C57BL/6 mice on day 18. (H) Schematic design of the experiment. (I) Histogram of Tox mRNA expression on five different CD8 T cell subpopulations (n = 5 per group). (J) Histogram of ratio T-bet/Eomes (mRNA) on five different CD8 T cell subpopulations (n = 4 per group). (K) Histogram of Klf4 mRNA expression on five different CD8 T cell subpopulations (n = 5 per group). All data are means ± SEM. Statistical analysis was performed using Student’s t test. ns (nonsignificant), P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001.