Fig. 3. The germline plays a key role in IGTA in zebrafish.

(A) Schematic representation of experimental setup and genetic crosses used to obtain wild-type offspring; red cell indicates a germline-specific mutation. (B) Relative mRNA levels of aldh1a2 and aldh1a3 in 24 hpf wild-type embryos from aldh1a2^+/+ incrosses (inx) and aldh1a2^rec/+ intercrosses (intx). Control expression levels were set at 1. n = 3 biologically independent samples. Data are means ± SD, and a two-tailed Student’s t test was used to calculate P values. Ct values are listed in table S1. (A) was created with BioRender.com.