. 2022 Nov 14;11:e81432. doi: 10.7554/eLife.81432

Table 4. Restraints used during energy minimization (EM) and stepwise equilibration (steps 1–6) of the asymmetric plasma membrane mimetic in kJ mol^-1nm^-2.

Step	Time [ns]	Timestep [fs]	Ensemble	Lipids	Dihedrals
EM				1000	1000
1	0.125	1	NVT	1000	1000
2	0.125	1	NVT	400	400
3	0.125	1	NPT	400	200
4	0.5	2	NPT	200	200
5	0.5	2	NPT	40	100
6	0.5	2	NPT	0	0

Table 4. Restraints used during energy minimization (EM) and stepwise equilibration (steps 1–6) of the asymmetric plasma membrane mimetic in kJ mol-1nm-2.