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. 2022 Nov 25;11:e83073. doi: 10.7554/eLife.83073

Figure 2. Impaired CD8+ T cell function in acid sphingomyelinase (Asm)-deficient tumor-bearing mice upon CD4+ T cell depletion.

Figure 2.

CD4+ T cells were depleted from Asm-WT and Asm-KO mice by repeated i.p. injection of anti-CD4 depleting antibody. Control groups received PBS. B16-F1 tumor cells were transplanted 1 day later subcutaneously. (A) Tumor volume was determined after tumor establishment based on caliper measurements (n=3–7). (B) Frequencies of CD4+ and CD8+ T cells in dLN and tumor and (C) expression of IFN-γ, CD44, and granzyme B of CD8+ tumor-infiltrating lymphocytes (TILs) were analyzed using flow cytometry. Representative dot plots are shown in the left panel. Results from two independent experiments are depicted as mean ± SEM. Statistical analysis was performed by two-way ANOVA with Sidak’s multiple comparisons or Student’s t-test. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Figure 2—source data 1. Impaired CD8+ T cell function in Asm-deficient tumor-bearing mice upon CD4+ T cell depletion.