Figure 1.

Hypoxia enhanced resistance of glioma cells to SAS-induced ferroptosis. (a) Cell viability curves at different concentrations of SAS for 24 h. Glioma cells under hypoxic environment became less sensitive to SAS. (b) Half-maximal inhibitory concentrations (IC₅₀) were calculated in each group. Hypoxic cells had an obvious higher IC₅₀ to SAS. (c) Lipid peroxidation assessed in the U87 and U251 cells after exposure to hypoxia, SAS, and Fer-1 by flow cytometry using C11-BODIPY. (d) Clone formation assay of cancer cells in the absence or presence of SAS or Fer-1 under normoxia or hypoxia. The bar graph showed mean ± SD of 3 independent experiments. ^∗P < 0.05 and ^∗∗P < 0.01.