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(A) PCR demonstrating the presence of the tlyC gene in TG (R. typhi and R. prowazekii) but not in SFG group (R. rickettsii, R. akari, and R. australis) rickettsiae. (B) Results showing dot blot hybridization on the five rickettsial DNA types in panel A, using a tlyC-specific probe. (The darker background for R. prowazekii is due to a longer exposure of the film to blot. Only one blot is represented here).
