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Western analysis of 10 μg of proteins extracted from E. coli. Nonhemolytic E. coli K-12 (lane 1), E. coli containing vector only (lane 2), and E. coli expressing R. typhi tlyC (lane 3) were incubated with the anti-GST antibody in conjunction with anti-goat IgG conjugate. GST-tlyC fusion protein was detected at 67 kDa.
