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Hemolytic activity of P. mirabilis BA6163 (wild type) and nonhemolytic WPM111/hpmA complemented with R. typhi tlyC. Each point is the geometric mean of three separate experiments. The hemolytic activity is defined as the reciprocal of the last dilution that results in complete hemolysis of the sheep erythrocytes. Nonhemolytic strain (P. mirabilis WPM111/hpmA) and P. mirabilis (WPM111) containing frameshift GFP-tlyC had no hemolytic activity.
