| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 95°C | 3 min | 1 |
| Denaturation | 95°C | 15 s | 35 |
| Annealing | 55°C | 30 s | |
| Extension | 72°C | 1 min | |
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | forever |
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