| Reagent | Amount |
|---|---|
| DNA template | 1μL |
| 2× M-PCR OPTI™ Mix (Dye Plus) | 10 μL |
| 10 μM PD-L1 primer 3 | 0.5 μL |
| 10 μM PD-L1 primer 4 | 0.5 μL |
| ddH2O | 8 μL |
| Total | 20 μL |
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