REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC anti-mouse CD3ε (1:250)	Biolegend	Cat # 100306, RRID: AB_312671
PE anti-mouse CD4 (1:200)	Biolegend	Cat # 100408, RRID: AB_312693
APC anti-mouse CD8a (1:200)	Biolegend	Cat # 100712, RRID: AB_312751
PE/Cyanine7 anti-mouse NK-1.1 (1:200)	Biolegend	Cat # 108713, RRID: AB_389363
PE anti-mouse CD279 (PD-1) (1:200)	Biolegend	Cat # 135205, RRID: AB_1877232
PE/Cyanine7 anti-mouse/human CD44 (1:200)	Biolegend	Cat # 103029, RRID: AB_830786
APC/Cyanine7 anti-mouse CD62L (1:200)	Biolegend	Cat # 104427, RRID: AB_830798
PE/Cyanine7 anti-mouse IFN-γ (1:100)	Biolegend	Cat # 505825, RRID: AB_1595591
Purified anti-mouse IFNAR-1	Biolegend	Cat # 127322, RRID: AB_11149116
TruStain FcX™ PLUS (anti-mouse CD16/32) (1:200)	Biolegend	Cat # 156603, RRID: AB_2783137
Chemicals, peptides, and recombinant proteins
Hydroxychloroquine sulfate (HCQ)	MedChemExpress	Cat # HY-B1370
Pentobarbital sodium	Sigma	Cat # 11715
Collagenase IV	YEASEN	Cat # 40510ES60
Deoxyribonuclease I (DNase I)	YEASEN	Cat # 10607ES15
Mouse Direct PCR Kit	Bimake	Cat # B40013
Agarose	Beyotime	Cat # ST004Q
YeaRed Nucleic Acid Gel Stain	YEASEN	Cat # 10202ES76
Fixation Buffer	Biolegend	Cat # 420801
Intracellular Staining Permeabilization Wash Buffer	Biolegend	Cat # 421002
Cell Stimulation Cocktail	Invitrogen	Cat # 00-4975-93
2× M-PCR OPTI™ Mix (Dye Plus)	Bimake	Cat # B45012
Dulbecco’s Modified Eagle Medium	Gibco	Cat # 11965118
RPMI 1640	Gibco	Cat # 11875176
Fetal bovine serum Trypsin	Gibco Gibco	Cat # 16000-044 Cat # 25200072
70 μm cell strainer	SPL life science	Cat # SPL-93070
Experimental models: Cell lines
LLC cell line	American Type Culture Collection	Cat # CRL-1642, RRID: CVCL_4358
Experimental models: Organisms/strains
Mouse: C57BL/6J PD-L1−/− (6–8 weeks, Male)	Shanghai Model Organisms Center	N/A
Oligonucleotides
PD-L1 primer 1	N/A	AGCTTTGGGTGTGGTTTAGTAGTA
PD-L1 primer 2	N/A	GGCTCTCCCCCTGAAGTTG
PD-L1 primer 3	N/A	GCTACTGCACCTGGCTTCTACCTG
PD-L1 primer 4	N/A	TACCTATCCCTGCACCATTGACCT
Software and algorithms
GraphPad Prism 8	GraphPad	N/A
FlowJo	Treestar	RRID: SCR_008520