Lysis buffer/Flag wash buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (1 M), pH 7.4 | 50 mM | 5 mL |
| NaCl | 150 mM | 0.8766 g |
| Triton X-100 | 0.5% | 0.5 mL |
| ddH2O | N/A | 94.5 mL |
| Total | N/A | 100 mL |
Store at 4°C; suggested maximum time for storage: 3 months.
Lysis buffer/Flag wash buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (1 M), pH 7.4 | 50 mM | 5 mL |
| NaCl | 150 mM | 0.8766 g |
| Triton X-100 | 0.5% | 0.5 mL |
| ddH2O | N/A | 94.5 mL |
| Total | N/A | 100 mL |
Store at 4°C; suggested maximum time for storage: 3 months.