Figure 1.
Post-PNI functional changes in the SDH
(A) Schematic diagram of in vivo patch-clamp recording from SG neurons at the L4 or L5 level. The ipsilateral hindlimb was stimulated with a brush (touch stimulus) or toothed forceps (pinch stimulus).
(B and C) RF map based on evoked EPSCs in response to the touch stimulus. The mean RF area was 0.42 ± 0.09 cm2 (n = 13 cells from 5 rats) in the Sham rats, 1.05 ± 0.16 cm2 (n = 19 cells from 10 rats) in the CCI-E rats, and 1.15 ± 0.14 cm2 (n = 18 cells from 8 rats) in the CCI-L rats. (Sham versus CCI-E: ∗∗p = 0.0090, versus CCI-L: ∗∗p = 0.027, One-way ANOVA).
(D) Representative chart recordings of the AUCs evoked by touch and pinch stimuli of the RF in Sham, CCI-E, and CCI-L rats.
(E) Comparison of AUC to touch stimulus. Sham: 1.15 ± 0.39 × 105 ΔpA/5 s, n = 13 cell from 5 rats; CCI-E: 3.48 ± 0.54 × 105 ΔpA/5 s, n = 23 cells from 12 rats, ∗p = 0.0342; CCI-L: 4.18 ± 1.05 × 105 ΔpA/5 s, n = 13 cells from 7 rats, ∗∗p = 0.0133. One-way ANOVA.
(F) Comparison of AUC to pinch stimulus. Sham: 1.19 ± 0.48 × 105 ΔpA/5 s, n = 10 cells from 5 rats; CCI-E: 4.23 ± 0.73 × 105 ΔpA/5 s, n = 23 cells from 12 rats, ∗p = 0.0188; CCI-L: 2.41 ± 0.79 × 105 ΔpA/5 s, n = 15 cells from 7 rats, p = 0.4861. One-way ANOVA. n.s., not significant.
(G) SG neurons were classified into 4 types according to their response to RF stimuli in in vivo patch-clamp recordings. Bars above the traces indicate the duration of the stimulation (5 s).
(H) Relative proportions of the 4 types of neurons among Sham, CCI-E, and CCI rats. The numbers in the graphs indicate the percentages. Sample sizes are indicated in parentheses. ∗p = 0.0489, Chi-squared test.