Figure 3.

Metabolic signature of synchronized NIH3T3 cells under hypoxic conditions, comparison between sham and tNMR treatment

(A–I) Levels of (A) glucose, (B) pyruvate, (C) lactate, (D) ADP/ATP ratio, (E) ATP, (F) ADP, (G) NAD+/NADH ratio, as well as (H) extracellular lactate and (I) overall lactate production after normoxic (black circles), hypoxic (red circles), as well as after combined treatment of tNMR and hypoxia (light blue circles) over two circadian cycles. Shown are means ± SEM, n = 4; open circles mark significant differences between normoxic and hypoxic sham treated cells, asterisks mark significant differences between sham treated normoxic cells and cell treated with tNMR under hypoxic conditions and open squares mark differences between hypoxic sham treated cells and hypoxic tNMR treated cells, calculated by two-way ANOVAs (GraphPad Prism 6.0).