Figure 4.

Increased NADP⁺/NADPH ratios and reduced G6PDH activities indicate a reduced flux through the PPP after tNMR treatment, while cell proliferation is increased

(A) NADP⁺/NADPH ratio under normoxic conditions.

(B) NADP⁺/NADPH ratio under hypoxic conditions.

(C) G6PDH activity under normoxic conditions.

(D) G6PDH activity under hypoxic conditions.

(E) Cell numbers after two and four days under normoxic conditions.

(F) Cell numbers after two and four days after hypoxic treatment; Black circles/bars represent sham treated cells, dark blue circles/bars represent ratios after treatment with tNMR, red circles/bars show levels of hypoxia sham treated cells and light blue circles/bars those of hypoxia and tNMR treated cells. Shown are means ± SEM, n = 4; asterisks mark significant differences between normoxic sham and tNMR treated cells, open circles mark significant differences between sham treated normoxic and hypoxic cells, open squares mark differences between hypoxic sham treated cells and hypoxic tNMR treated cells, calculated by two-way ANOVAs (GraphPad Prism 6.0).