SCW phenotypes of PHYB-OE and PIF4-OE plants.
(A) Transmission electron micrographs of inflorescence stem cross sections of plants grown in WL. If, interfascicular fiber cell; V, vessel cells. Scale bar, 5 μm.
(B) Measurements of SCW thickness of cells in (A). Data were collected from three biological replicates, and more than 10 cells were measured per biological replicate. Student’s t-test (∗∗P < 0.01) was used for statistical analysis, mean ± SD.
(C) Tensile strength of inflorescence stems; the WT stem tensile strength was set to 1. Student’s t-test (∗∗P < 0.01) was used for statistical analysis, n = 15, mean ± SD.
(D) Lignin content in inflorescence stems. Student’s t-test (∗P < 0.05) was used for statistical analyses, n = 3, mean ± SD.
(E) Cellulose content in inflorescence stems. Student’s t-test (∗∗P < 0.01, ∗P < 0.05) was used for statistical analyses, n = 3, mean ± SD.
(F) Expression of SCW regulatory (NST1 and SND1) and biosynthesis-related (4CL1 and IRX8) genes in different genotypes grown under WL. Three biological replicates were performed. Student’s t-test (∗∗P < 0.01, ∗P < 0.05) was used for statistical analysis, mean ± SD.