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. 2022 Aug 4;3(6):100416. doi: 10.1016/j.xplc.2022.100416

Figure 5.

Figure 5

PIF4 represses MYC2 transcriptional activity.

(A) Schematic representation of the NST1 promoter-driven dual-LUC reporter gene and three effector gene constructs. 35S promoter, NST1 promoter (−1 to −3711 bp from ATG), Renilla luciferase (REN), and firefly luciferase (LUC) are indicated in reporter constructs. In effector constructs, PIF4, PIF5, and MYC2 are driven by the 35S promoter.

(B) PIF4/PIF5 inhibit MYC2 activation of the NST1 promoter. Arabidopsis protoplasts were transfected with the reporter constructs in combination with different effector constructs. After transfection, the protoplasts were kept in the dark for 16 h. Relative luminescence was normalized to that of protoplasts transformed with the reporter and empty effector (GFP). Tukey’s honestly significant difference (HSD) test (∗∗P < 0.01) was used for statistical analysis, n = 3, mean ± SD.

(C) Subcellular localization of PIF4 and MYC2/MYC4. Constructs of PIF4-CFP, MYC2-YFP, and MYC4-YFP were transferred to tobacco leaves, separately or together, by agroinfiltration. The tobacco leaves were then kept in the dark for 12 h before fluorescence observation. Scale bar, 5 μm.