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. 2022 Aug 4;3(6):100416. doi: 10.1016/j.xplc.2022.100416

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

PIF4 affects MYC2 stability in the dark and in far-red light. MYC2 stability was examined in the WT and pifq backgrounds.

(A) Transgenic plants (WT/MYC2-YFP and pifq/MYC2-YFP) were grown in WL conditions for 10 days and then exposed to dark conditions. MYC2 and ACTIN were immunoblotted. Normalized MYC2 abundance relative to ACTIN is shown as MYC2/ACTIN.

(B) Transgenic plants (WT/MYC2-YFP and pifq/MYC2-YFP) were grown in WL conditions for 10 days and then exposed to far-red light. MYC2 and ACTIN were immunoblotted. Normalized MYC2 abundance relative to ACTIN is shown as MYC2/ACTIN.

(C) Transgenic plants (WT/MYC2-YFP and pifq/MYC2-YFP) were grown in WL conditions for 4 days and then treated with/without darkness for 30 h. YFP fluorescence was observed in the root tip, and the relative change in signal intensity was quantified. Scale bar, 50 μm.