TABLE 1.

Effect of S. aureus on TFA of ECs^a

Time of culture (h)	TFA (mU of FXa/2 × 105 ECs) for:
	S. aureusb	Cell-associated S. aureusc	Intracellular S. aureusd
0 (no bacteria)	1.52 ± 0.65e	0.70 ± 0.43	0.79 ± 0.33
5	3.33 ± 1.59	0.70 ± 0.37	0.88 ± 0.41
6	6.39 ± 1.32	0.92 ± 0.78	0.92 ± 0.46
7	6.83 ± 1.17	0.70 ± 0.57	0.96 ± 0.49
8	6.48 ± 1.75	0.92 ± 0.79	0.96 ± 0.41

^aMonolayers of 2 × 10⁵ ECs were incubated with 4 × 10⁷ S. aureus strains for the indicated periods of time. All values represent the means ± standard deviations of at least three individual experiments with ECs from different donors. 

^bValues of continuous incubation with S. aureus (Fig. 3A). 

^cECs were incubated with 4 × 10⁷ S. aureus organisms for 1 h, washed with warm PBS to remove non-cell-associated bacteria, and then cultured for the indicated additional periods of time. 

^dECs were incubated with 4 × 10⁷ S. aureus organisms for 1 h and then washed, treated with lysostaphin to lyse extracellular but not intracellular bacteria, washed, and cultured for the indicated additional periods of time. 

^eThe value of P was <0.001 for S. aureus at 0 h versus S. aureus at 6, 7, and 8 h.