TABLE 2.
Influence of bacterial viability, bacterial fragments, and bacterium-derived factors on TFA of ECsa
| Time of incubation (h) | TFA (mU of FXa/2 × 105 ECs) for:
|
|||
|---|---|---|---|---|
| Medium | Live S. aureus | UV-killed S. aureus | Supernatant from S. aureus culture | |
| 7 | NDb | 4.95 ± 3.27 | 2.47 ± 1.69 | 2.58 ± 2.69 |
| 24 | 0.73 ± 0.79c | 8.68 ± 4.09 | 1.78 ± 2.65 | 0.76 ± 0.79 |
a
Monolayer of 2 × 105 ECs were incubated with M199 and 10% HuSi (medium), 5 × 107 live or UV-killed S. aureus organisms, or supernatants from overnight S. aureus cultures that were passed through a 0.45-μm-pore-size filter. After 7 or 24 h of incubation, ECs were washed and endothelial TFA was measured. Values represent means of three to five individual experiments with ECs from different donors.
b
ND, not determined, but values are expected to be similar to those at 24 h.
c
The value of P was <0.05 for medium at 24 h versus live S. aureus at 7 and 24 h.