TABLE 3.

Effect of endogenous IL-1 on EC TFA^a

Stimulus	rIL-1ra	TFA (mU of FXa/2 × 105 ECs) at the following timeb
		7 h	24 h
None	−	0.74 ± 0.63	0.74 ± 0.63
	+	4.28 ± 3.91	1.82 ± 1.94
S. aureus	−	6.45 ± 0.33	14.82 ± 5.38
	+	8.11 ± 2.55	14.03 ± 5.01
IL-1	−	56.06 ± 8.00	12.69 ± 5.84
	+	17.74 ± 10.28c	8.37 ± 5.69

^aTreatment of ECs: 2 × 10⁵ ECs were incubated at 37°C with M199 plus 10% HuSi (none) or 5 × 10⁷ S. aureus organisms in the absence (−) or presence (+) of 30 ng of rIL-1ra per ml for 7 or 24 h. Control monolayers were stimulated with 5 ng of IL-1 per ml. Then ECs were washed, and TFA was determined as described in Material and Methods. 

^bValues represent means ± standard deviations of at least four experiments with ECs from different donors. 

^cThe value of P was 0.03 for the TFA with IL-1 as the stimulus in the presence of rIL-1ra at 7 h versus that in the absence of rIL-1ra at 7 h.