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. 2022 Nov 23;3(4):101884. doi: 10.1016/j.xpro.2022.101884

PCR cycling conditions

Steps Temperature Time Cycles
Initial Denaturation 95°C 10 min 1
Denaturation 95°C 15 s 40 cycles
Annealing 60°C 60 s
Denaturation 95°C 15 s 1 [Melt Curve Stage]
Annealing 60°C 60 s
Denaturation 95°C 15 s