REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Gelatin	FUJIFILM Wako Pure Chemical Corporation	Cat# 190-15805
Y-27632	FUJIFILM Wako Pure Chemical Corporation	Cat# 257-00511
KLC-TM medium	KaLy-Cell	Cat# KLC-TM
KLC-SM medium	KaLy-Cell	Cat# KLC-SM
Hepatocyte basal medium	Lonza	Cat# CC-3199
Primate ES Cell Medium	ReproCELL	Cat# RCHEMD001
Freezing medium for human ES/iPS cells (DAP213)	ReproCELL	Cat# RCHEFM001
Recombinant human bFGF (FGF2)	ReproCELL	Cat# RCHEOT002
D-PBS(-)	Nacalai Tesque	Cat# 11482-15
0.05% trypsin/EDTA	Thermo Fisher Scientific	Cat# 25300054
DMEM	Wako	Cat# 043-30085
Fetal bovine serum	Thermo Fisher Scientific	Cat# 10270-106
CytoTune-iPS 2.0	Medical & Biological Laboratories Co., Ltd.	Cat# IDT-DV0304
Penicillin‒streptomycin solution (×100)	FUJIFILM Wako Pure Chemical Corporation	Cat# 16823191
Hanks’ Balanced Salt Solution (HBSS)	Life Technologies	Cat# 14025092
Critical commercial assays
SuperPREP II Cell Lysis & RT Kit for quantitative PCR	TOYOBO CO., LTD.	Cat# SCQ-401
Luna Universal qPCR Master Mix	New England Biolabs Inc.	Cat# M3003E
TaqMan Array 96-Well FAST Plate(Human Stem Cell Pluripotency)	Applied Biosystems	Cat# 4418722
TaqMan™ Fast Advanced Master Mix	Thermo Fisher Scientific	Cat# 4444963
Experimental models: Cell lines
Cryopreserved Hepatocytes Species:Human, Lot#S1412T, Lot#S1238 and Lot#S1350	KaLy-Cell	Cat# HHCPC-2 M
hiPSC Lines 201B7	CiRA Foundation	N/A
MEF cells	ReproCELL Inc.	Cat# RCHEFC003
Oligonucleotides
human OCT3/4 forward, GACAGGGGGAGGGGAGGAGCTAGG, human OCT3/4 reverse, CTTCCCTCCAACCAGTTGCCCCAAAC,	Takahashi et al.2	N/A
human SOX2 forward, GGGAAATGGGAGGGGTGCAAAAGAGG, human SOX2 reverse, TTGCGTGAGTGTGGATGGGATTGGTG,	Takahashi et al.2	N/A
human KLF4 forward, TGATTGTAGTGCTTTCTGGCTGGGCTCC, human KLF4 reverse, ACGATCGTGGCCCCGGAAAAGGACC,	Takahashi et al.2	N/A
human c-MYC forward, GCGTCCTGGGAAGGGAGATCCGGAGC, human c-MYC reverse, TTGAGGGGCATCGTCGCGGGAGGCTG,	Takahashi et al.2	N/A
human NANOG forward, CAGCCCCGATTCTTCCACCAGTCCC, human NANOG reverse, CGGAAGATTCCCAGTCGGGTTCACC,	Takahashi et al.2	N/A
human GDF3 forward, CTTATGCTACGTAAAGGAGCTGGG, human GDF3 reverse, GTGCCAACCCAGGTCCCGGAAGTT,	Takahashi et al.2	N/A
human REX1 forward, CAGATCCTAAACAGCTCGCAGAAT, human REX1 reverse, GCGTACGCAAATTAAAGTCCAGA,	Takahashi et al.2	N/A
human DNMT3b forward, TGCTGCTCACAGGGCCCGATACTTC, human DNMT3b reverse, TCCTTTCGAGCTCAGTGCACCACAAAAC,	Takahashi et al.2	N/A
human GAPDH forward, ACCACAGTCCATGCCATCAC, human GAPDH reverse, TCCACCACCCTGTTGCTGTA,	NCBI Reference Sequence	NM 004048
human β-ACTIN forward, CAACCGCGAGAAGATGAC, human β-ACTIN reverse, AGGAAGGCTGGAAGAGTG,	Kajihara et al.3	N/A
human HNF1β forward, CTGACTACCAGCTAACTCCAGTCTC, human HNF1β reverse, GACTGCAACTTTTTCTTCTGCTATC,	NCBI Reference Sequence	NM_000458.3
human HNF4α forward, GAACAGGAGCTCTTAACTACAGTGG, human HNF4α reverse, CTGTCAAGAGTCATGAATTCTCCTT,	NCBI Reference Sequence	NM_000457.4
human β-ACTIN forward, TGGCACCCAGCACAATGAA, human β-ACTIN reverse, CTAAGTCATAGTCCGCCTAGAAGCA,	NCBI Reference Sequence	NM_001101
Other
LightCycler 96 Real-Time PCR system	Roche	Cat# 05 815 916 001
InvitrogenTM EVOSTM FL Auto Imaging System	Thermo Fisher Scientific	Cat# AMAFD1000
C.B-17/Icr-scid/scidJcl, male, 8 week-old	CLEA Japan	N/A