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. 2022 Nov 23;10:e14338. doi: 10.7717/peerj.14338

Table 1. Forward and reverse primers used for real-time qPCR for 17 developmental and two reference genes (*).

Gene Forward primer sequence (5′→ 3′) Reverse primer sequence (5′→ 3′) Accession number
runx2a TGTGGCTATGGCGTCTAACA ATCTCCACCATGGTCCGGT NM_212858.2
runx2b GGGCCAAACGCAGATTACAG TCTGTCGAACCTGGAAGACG NM_212862.2
sp7 GGATACGCCGCTGGGTCTA TCCTGACAATTCGGGCAATC NM_212863.2
col1a1a TTGCTTAGACCTGCGCTTCA CCAGGGGGATTTTACACGCT NM_199214.1
col1a2 GCTGGCCTTCATGCGTCTAC ACACAGCCTTCTTCAGGTTTCC NM_182968.2
col2a1 GCGACTTTCACCCCTTAGGA TGCATACTGCTGGCCATCTT NM_131292.1
col9a1a GGGGTGCGGTTGGATTTACT TCCTGTCGATCCTTTCTCGC NM_001130624.1
col9a1b AGACAAGTGTGCATGCGAGT AAACCACTGTCACCTTGGGG NM_213264.2
dlx2a GACTCAGTATCTGGCCTTGC CTGCTCGGGTGGGATCTCT NM_131311.2
nkx3.2 ACGCTAAAGCGCAAATCGAC TTACAGTCGGACACGCAGTC NM_178132.2
fgf3 GGTGGCAATCAAGGGACTGT TGGTGCCGTGATGCATAAGT NM_131291.1
fgf8a GCCGTAGACTAATCCGGACC TTGTTGGCCAGAACTTGCAC NM_131281.2
fgfr1a ATCTGTGTGATGGTGGGGAC GATGAGCTGGAGTCCACAGAC NM_152962.3
fgfr1b AGCATGGCTGACCGTTGTTA GAGCTGGACTCCACTGACAC NM_001161732.1
fgfr2 GCCTGTATGGTGGTGATCGT TCTGACGACACTGTTACCTGG NM_001243004.1
fgfr3 TCTCCGTGGTGAGGAAAGTC TGCCATACAATGGGAAGGAGG NM_131606.2
fgfr4 CTCTTGGACGTGTTGGAACG CCACTGGATGTGAGGCTGAG NM_131430.1
elf1a* CTGGAGGCCAGCTCAAACAT TCAAGAAGAGTAGTACCGCTAGCATTAC NM_131263.1
rpl13* TCTGGAGGACTGTAAGAGGTATGC AGACGCACAATCTTGAGAGCAG NM_212784