Table 1. Forward and reverse primers used for real-time qPCR for 17 developmental and two reference genes (*).
Gene | Forward primer sequence (5′→ 3′) | Reverse primer sequence (5′→ 3′) | Accession number |
---|---|---|---|
runx2a | TGTGGCTATGGCGTCTAACA | ATCTCCACCATGGTCCGGT | NM_212858.2 |
runx2b | GGGCCAAACGCAGATTACAG | TCTGTCGAACCTGGAAGACG | NM_212862.2 |
sp7 | GGATACGCCGCTGGGTCTA | TCCTGACAATTCGGGCAATC | NM_212863.2 |
col1a1a | TTGCTTAGACCTGCGCTTCA | CCAGGGGGATTTTACACGCT | NM_199214.1 |
col1a2 | GCTGGCCTTCATGCGTCTAC | ACACAGCCTTCTTCAGGTTTCC | NM_182968.2 |
col2a1 | GCGACTTTCACCCCTTAGGA | TGCATACTGCTGGCCATCTT | NM_131292.1 |
col9a1a | GGGGTGCGGTTGGATTTACT | TCCTGTCGATCCTTTCTCGC | NM_001130624.1 |
col9a1b | AGACAAGTGTGCATGCGAGT | AAACCACTGTCACCTTGGGG | NM_213264.2 |
dlx2a | GACTCAGTATCTGGCCTTGC | CTGCTCGGGTGGGATCTCT | NM_131311.2 |
nkx3.2 | ACGCTAAAGCGCAAATCGAC | TTACAGTCGGACACGCAGTC | NM_178132.2 |
fgf3 | GGTGGCAATCAAGGGACTGT | TGGTGCCGTGATGCATAAGT | NM_131291.1 |
fgf8a | GCCGTAGACTAATCCGGACC | TTGTTGGCCAGAACTTGCAC | NM_131281.2 |
fgfr1a | ATCTGTGTGATGGTGGGGAC | GATGAGCTGGAGTCCACAGAC | NM_152962.3 |
fgfr1b | AGCATGGCTGACCGTTGTTA | GAGCTGGACTCCACTGACAC | NM_001161732.1 |
fgfr2 | GCCTGTATGGTGGTGATCGT | TCTGACGACACTGTTACCTGG | NM_001243004.1 |
fgfr3 | TCTCCGTGGTGAGGAAAGTC | TGCCATACAATGGGAAGGAGG | NM_131606.2 |
fgfr4 | CTCTTGGACGTGTTGGAACG | CCACTGGATGTGAGGCTGAG | NM_131430.1 |
elf1a* | CTGGAGGCCAGCTCAAACAT | TCAAGAAGAGTAGTACCGCTAGCATTAC | NM_131263.1 |
rpl13* | TCTGGAGGACTGTAAGAGGTATGC | AGACGCACAATCTTGAGAGCAG | NM_212784 |