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. 2022 Oct 21;41(48):5186–5198. doi: 10.1038/s41388-022-02507-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A RT–qPCR results of OTUB1 mRNA expression in AGS (left panel) or MKN28 (right panel) cell line transfected with scrambled or two independent siRNA targeting OTUB1 for 48 h, respectively. B Western blot results showing the expression level of YAP and OTUB1 in AGS (left panel) or MKN28 (right panel) cell line transfected with scrambled or two independent siRNA targeting OTUB1 for 48 h, respectively. C RT–qPCR results of CTGF and CYR61 mRNA expression in AGS (left panel) or MKN28 (right panel) cell line transfected with scrambled or two independent siRNA targeting OTUB1 for 48 h, respectively. D Transcriptional activity of TEAD measured by luciferase assay with a reporter containing tandem TEAD-binding sites in AGS (left panel) or MKN28 (right panel) cell line transfected with scrambled or two independent siRNA targeting OTUB1 for 48 h, respectively. E RT–qPCR results of OTUB1 mRNA expression in MKN28 cell line stable expressing OTUB1-flag or lentiviral vector. F Western blot results showing the expression level of YAP and OTUB1 in MKN28 cell line stable expressing OTUB1-flag or lentiviral vector. G RT–qPCR results of CTGF and CYR61 mRNA expression in MKN28 cell line stable expressing OTUB1-flag or lentiviral vector. H Transcriptional activity of TEAD measured by luciferase assay with a reporter containing tandem TEAD-binding sites in MKN28 cell line stable expressing OTUB1-flag or lentiviral vector. I Immunohistochemistry (IHC) detecting OTUB1 and YAP expression in gastric cancer specimen. J Chi-square test to determine the correlation between OTUB1 and YAP. Data are normalized to the values of the control group and are presented as mean ± SEM. One-way ANOVA is conducted to detect significant differences in panel A, C, D. The t-test is conducted to detect significant differences in panel E, G, H (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).