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. 2022 Aug 6;15(5):467–478. doi: 10.1007/s12195-022-00730-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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BECs and LECs express unique endothelial surface markers. Representative flow cytometry diagrams demonstrating unique endothelial surface markers for PDPN and CD31. (a) BEC (92.7%) showing CD31⁺ and PDPN⁻, while (b) LEC showing CD31⁺ and PDPN⁺. Representative flow cytometry histograms indicating (c and d) BEC and (e and f) LEC stained with CD31-PE (in black), PDPN-FITC (in black), and isotype controls (in grey). (g) Real-time quantitative qRT-PCR was used to analyze PDPN expression for LEC and BEC relative to GAPDH. Data represents mean $\pm$ SD, n = 4 per group, ***p < 0.001. All p values were determined by unpaired t tests.