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. 2022 Aug 6;15(5):467–478. doi: 10.1007/s12195-022-00730-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The roles of FLCN and PDPN on cord-like networks formation. (a and b) Luciferase knockout BEC (rBEC ^ΔLuc) or (c and d) FLCN knockout BEC (rBEC^ΔFLCN) stained in red are encapsulated in 3D fibrin gels together with (a and c) Luciferase knockout LEC (gLEC ^ΔLuc) or (b and d) PDPN knockout LEC (rLEC^ΔPDPN) stained in green. Representative images of CLS formation were imaged at 48 h. Scale bars are 500 μm. (e and f) CLS formed on 3D fibrin gel was quantified for tube length using AutoTube. Data represents mean $\pm$ SD, n = 4 per group, n.s. p > 0.05, *p < 0.05. All p values were determined by ANOVA followed by post hoc testing with Tukey analysis.