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. 2022 Aug 6;15(5):467–478. doi: 10.1007/s12195-022-00730-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The roles of FLCN and PDPN on cell migration. Representative images of the wound healing assay taken at t = 16, 35, and 48 h for (a) BEC ^ΔLuc (in red): BEC ^ΔLuc (in green), (b) LEC ^ΔLuc (in red): LEC ^ΔLuc (in green), (c) BEC ^ΔLuc (in red): LEC ^ΔLuc (in green), (d) BEC^ΔFLCN (in red): LEC ^ΔLuc (in green), and (e) BEC^ΔLuc (in red): LEC ^ΔPDPN (in green). Each well was seeded with 7000 cells and allowed to settle for 24 h, then imaged for up to 48 h. Scale bars are 500 μm. Overlap scores for (f) wild-type BEC and LEC, as well as (g) knock-out BEC and LEC. The LEC-BEC condition consists of 4 replicates and the same and knockout conditions consist of 4 replicates from either of the two conditions. Data represents mean $\pm$ SD, n = 4 per group, n.s. p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001. All p values were determined by ANOVA followed by post hoc testing with Tukey analysis.