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. 2022 Aug 22;68(5-6):619–644. doi: 10.1007/s00294-022-01251-1

Table 2.

Plasmids used in this study

Plasmid Description Sources or References
pRS316 lc URA3 cloning vector (Sikorski and Hieter 1989)
pRS315 lc LEU2 cloning vector (Sikorski and Hieter 1989)
pRS425 hc LEU2 cloning vector (Sikorski and Hieter 1989)
pPM2 lc URA3 RPL33A in pRS316 (Martin-Marcos et al. 2007)
pPM40 lc URA3 rpl33a-Y44R in pRS316 This study
pPM41 lc URA3 rpl33a-G69R in pRS316 This study
pPM42 lc URA3 rpl33a-G76R in pRS316 This study
pPM43 lc URA3 rpl33a-G76R, G79R in pRS316 This study
pPM44 lc URA3 rpl33a-L102R in pRS316 This study
pPM45 lc URA3 rpl33a-∆99–107 in pRS316 This study
pPM46 lc URA3 rpl33a-A102-105 in pRS316 This study
pPM47 lc URA3 rpl33a-A102-107 in pRS316 This study
pPM17 lc LEU2 RPL33A in pRS315 This study
pPM48 lc LEU2 rpl33a-Y44R in pRS315 This study
pPM49 lc LEU2 rpl33a-G69R in pRS315 This study
pPM50 lc LEU2 rpl33a-G76R in pRS315 This study
pPM51 lc LEU2 rpl33a-G76R, G79R in pRS315 This study
pPM52 lc LEU2 rpl33a-L102R in pRS315 This study
pPM53 lc LEU2 rpl33a-∆99–107 in pRS315 This study
pPM54 lc LEU2 rpl33a-A102-105 in pRS315 This study
pPM55 lc LEU2 rpl33a-A102-107 in pRS315 This study
pPM56 hc LEU2 RPL33A in pRS425 This study
pPM57 hc LEU2 rpl33a-Y44R in pRS425 This study
pPM58 hc LEU2 rpl33a-G69R in pRS425 This study
pPM59 hc LEU2 rpl33a-G76R in pRS425 This study
pPM60 hc LEU2 rpl33a-G76R, G79R in pRS425 This study
pPM61 hc LEU2 rpl33a-L102R in pRS425 This study
pPM62 hc LEU2 rpl33a-∆99–107 in pRS425 This study
pPM63 hc LEU2 rpl33a- A102-105 in pRS425 This study
pPM64 hc LEU2 rpl33a-A102-107 in pRS425 This study
pPM65 lc LEU2 rpl33a-L7R in pRS315 This study
pPM66 lc LEU2 rpl33a-∆L29 in pRS315 This study
pPM67 lc LEU2 rpl33a-V35R in pRS315 This study
pPM68 lc LEU2 rpl33a-F43R in pRS315 This study
pPM69 lc LEU2 rpl33a-G79R in pRS315 This study
pPM70 lc LEU2 rpl33a-Y103R in pRS315 This study
pPM71 lc LEU2 rpl33a-A40-44 in pRS315 This study
pPM72 lc LEU2 rpl33a-A92-99 in pRS315 This study
pPM73 lc LEU2 rpl33a-A104-107 in pRS315 This study
p180 sc URA3 GCN4-lacZ with WT leader (Hinnebusch 1985)
p226 sc URA3 GCN4-lacZ with uORF4 only (Mueller and Hinnebusch 1986)
pM226 sc URA3 GCN4‐lacZ with uORF1 extending into GCN4 (Grant et al. 1994)
p227 sc URA3 GCN4-lacZ uORF-less (Mueller and Hinnebusch 1986)
p367 sc URA3 HIS4(ATG)-lacZ (Donahue and Cigan 1988)
p391 sc URA3 HIS4(TTG)-lacZ (Donahue and Cigan 1988)
YCplac22 sc TRP1 cloning vector (Gietz and Sugino 1988)
p4280/YCpSUI3-S264Y-W sc TRP1 SUI3-S264Y in YCplac22 (Valasek et al. 2004)
YEp112 hc TRP1 cloning vector (Gietz and Sugino 1988)
pPM77 hc TRP1 SUI1 This study
pFA6MX4 kanMX4 (Wach et al. 1994)
pAG25 natMX4 (Goldstein and McCusker 1999)
pUG6 loxP–kanMX–loxP (Guldener et al. 1996)
pAGH1 loxP–natMX4–loxP This study