Table 10.
Flow cytometry panels C and D.
| Antibody/probe | Fluorochrome | Clone | Remarks |
|---|---|---|---|
| CD11c | Alexa647 | 3.9 | |
| CD123 | PerCP-Cy5.5 | 7G3 | |
| CD14 | Pacific Blue | M5E2 | |
| CD16 | Alexa700 | 3G8 | |
| CD20 | FITC | L27 | |
| CD3 | FITC | SP34 | |
| CD45 | APC-Cy7 | D058-1283 | |
| CD8 | FITC | SK-1 | |
| CD80 | PE-Cy7 | L307.4 | Panel C only |
| CD83 | PE | HB15e | Panel C only |
| CD86 | PECF594 | Fun-1 (2331) | Panel C only |
| CD80 IgG1K | PE-Cy7 | MOPC21 | Panel D only |
| CD83 IgG1 | PE | MOPC21 | Panel D only |
| CD86 IgG1 | PECF594 | X40 | Panel D only |
| HLA-DR | V500 | G46-6 | |
| Fc Block | N/A | N/A | E23, E24, E25 only |
| Live/Dead Fixable | Green | N/A | E23, E24, E25 only |