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. 2022 Nov 24;13(11):994. doi: 10.1038/s41419-022-05411-6

Fig. 2. Concomitant upregulation of MYC and MET in a subset of hepatocytes triggers tumorigenesis in mice.

Fig. 2

A Scheme illustrating the protocol of hydrodynamic tail vein injection used in R26stopMet mice with Cre, Myc, or Cre + Myc plasmids. B Liver representative photographs of hydrodynamically injected R26stopMet mice with Myc or Cre + Myc plasmids. C Pie charts representing the percentage of R26stopMet mice with or without tumours (black and white, respectively) after hydrodynamic injection of Cre, Myc, or Cre + Myc plasmids. D, E Dot plots representing the number of tumours per mouse (D) and the tumour weight (E) in R26stopMet mice hydrodynamically injected with Cre, Myc, or Cre + Myc plasmids. F Dot plots reporting the mRNA expression levels of Myc and Mettg analysed by RT-qPCR in dissected Alb-R26Met and Myc-R26Met tumours, normalised using the Gapdh housekeeping gene and expressed as RQ. Statistical analyses were performed by Mann–Whitney. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.