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. 2022 Nov 24;13(11):994. doi: 10.1038/s41419-022-05411-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Representative haematoxylin and eosin staining of frozen, fixed Alb-R26^Met and Myc-R26^Met tumours and adjacent livers. B, C Representative images (B) and graph with quantifications (C) of Ki67 immunofluorescence staining of Alb-R26^Met and Myc-R26^Met tumour sections (scale bar: 50 µm). D–G mRNA expression levels by RT-qPCR of the proliferation marker Mki67 (D), of HCC markers Afp, Spp1, Gpc3, and Epcam (E), of the non-histone chromatin protein Hmga1 (F), and of mesenchymal Vim and epithelial Cdh1 markers (G) in Myc-R26^Met versus Alb-R26^Met tumours. H Heatmap reporting the RQ differential expression of all genes evaluated by RT-qPCR in this study. Values were normalised with the Gapdh housekeeping gene and expressed as RQ, all values relative to Alb-R26^Met tumours. Statistical analyses were performed by Mann–Whitney. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.