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. 2022 Nov 24;13:7216. doi: 10.1038/s41467-022-34865-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a1–a3 Contrast-enhanced ex vivo µCT of two different anterior midgut regions (smaller diameter and larger diameter) and with a 3D volume rendering (Supplementary Video 5). b Axial in vivo μMRI of a M. sexta L5 larva 10 min after the injection of contrast agent (CA). The gut wall shows the strong uptake of CA. c PTA-stained midgut in µCT, d Mean CT (n = 25, voxel size of 350 µm) and µCT (n = 10, voxel size of 7.55 µm) gut wall thicknesses from the complete midgut with descriptive statistics. The fat body of M. sexta in μMRI with (f) and without (e) fat suppression. The difference between e and f is shown in g and corresponds to the fat body of M. sexta (h). i Shows an axial paraffin section (L5d2), which shows the fat body and confirms our analysis of e and f. j This shows a corresponding axial μMRI of an M. sexta (L5d5) larvae. Bar charts represent mean and SD. Every data point represents a single animal. Source data are provided as a Source Data file.