Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 25;5:1292. doi: 10.1038/s42003-022-04241-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a GFP-SET8 immunoprecipitation in overexpressed GFP-SET8 FL or GFP-SET8M with HA-Ubiquitin in COS-7 cells. Western blots detecting the amount of ADP-ribosylation (top panel), Ubiquitination (middle panel), and GFP fusion protein levels (bottom panel) in GFP immunoprecipitates. The fold increase was calculated by densitometry and indicated at the bottom of the western blot. b Cycloheximide chase analysis of SET8 stability in GFP-SET8 FL or GFP-SET8 M in HeLa cells. Western blots detecting the amount of GFP-SET8 FL and GFP-SET8 M protein levels with their respective actin levels (control) during cycloheximide time course (top panel). Respective densitometry analyses of GFP/Actin ratio representative of at least 2 biological experiments (bottom panel). c Western blots (left side) detecting the amount of PARP1 (top), SET8 protein (middle) as well as the amount of H4K20me1, H4K20me2, and H4K20me3 levels (bottom) in total protein extract of knockdown HeLa cells treated with siRNA (esiRNA) for GFP (control), esiRNA PARP1 and esiRNA SET8, respectively. Respective densitometry analyses of protein abundance representative of at least 2 biological experiments are shown (right side; n = 2). Ponceau stain was used as control (left side).