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. 2022 Nov 25;13:7269. doi: 10.1038/s41467-022-34846-w

Fig. 1. Phenotypical characterisation and secretory profiling of different AT depots.

Fig. 1

a Experimental workflow showing anatomical localisation of AT depots and subsequent procedures performed on AT explants. b Upper panel: H&E stainings of tissue sections. Lower panel: immunofluorescence images of AT sections stained for perilipin and UCP1. Nuclei are counterstained with DAPI. Scale bars: 50 µm. Insets show higher magnifications of randomly selected areas. Representative pictures of 1 out of 2 replicates. c Western blot of representative AT lysates (2 out of 4 replicates) stained for perilipin and UCP1. A brain lysate was loaded as negative control for AT-specific proteins. d Western blot of representative AT lysates (1 out of 2 replicates) and corresponding supernatants stained for β-actin, adiponectin, perilipin and UCP1. e, f Unsupervised hierarchical clustering (heatmap, e) and principal component analysis (f) of the 363 consistently identified secreted proteins detected by MS in the conditioned media of different AT depots. Hierarchical clustering was performed using the complete agglomeration method, Z-score-scaled abundances are displayed. N = 6 biological replicates per group. AT adipose tissue, AR PVAT aortic arch perivascular AT, TH PVAT thoracic PVAT, AB PVAT abdominal PVAT, VI WAT visceral white AT, SC WAT subcutaneous white AT, BAT interscapular brown AT, UCP1 uncoupling protein 1, M molecular weight marker.