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. 2022 Nov 25;13:7269. doi: 10.1038/s41467-022-34846-w

Fig. 5. Local effect of AT-conditioned media on cultured sympathetic neurons.

Fig. 5

a Schematic cartoon showing the structure of microfluidic devices employed for sympathetic neuronal cultures. b Representative images of SCG sympathetic neurons seeded on microfluidic devices, with the axonal chamber incubated for 24 h with either wt or ob/ob fat explants conditioned media (C.M.) showing brightfield appearance (BF, left panel) and β3-tubulin staining (IF, right panel) of sympathetic neurites. Arrows point at axonal swellings. Scale bars: 20 µm. Bar chart shows the quantification of axonal swellings normalised on total axonal length. Data are shown as mean ± SEM of 39 and 35 random fields acquired from 3 independent experiments and analysed by unpaired two-tailed independent t-test (unequal distribution), p = 2.98E-06. c, d Western blots showing TYR.H. abundances in wt and ob/ob hearts (c) and PVAT-denuded aortas (d) lysates and relative quantifications. N = 5 biological replicates, unpaired two-tailed independent t-test (unequal distribution). Data are shown as mean ± SEM. SCG superior cervical ganglia, TH thoracic PVAT, AR aortic arch PVAT, TYR.H. tyrosine hydroxylase, M molecular weight marker. Source data are provided as a Source Data file.