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. 2022 Nov 24;22:443. doi: 10.1186/s12890-022-02209-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MiR-671-5p is regulated by circ_0017109. A Predicted miR-671-5p target sequences in circ_0017109 sequence based on CircInteractome database. Luciferase activity of wide-type or mutated circ_0017109 reporter was determined in H293K cells in the presence of miR-NC or miR-671-5p mimic. B RNA-pull down assay of circ_0017109 enrichment using biotinylated miR-671-5p or biotin-NC. C RNA-immunoprecipitation assay using anti-AGO2 antibody or nonspecific rabbit IgG. ***, P < 0.001