Table 2.

Oligonucleotides used in this study

Target/purpose	Sequence	Source
Primers for mutagenesis
Deletion of E. coli MP1 lldD	5′-GCTTCTTCTAGAGGTACCGCATGGTGCTGCTCAGTCGACGTG-3′ 5′-CCCTTAAGCTTCATGCGTTTTTCTCCCTCG-3′ 5′- AAACGCATGAAGCTTAAGGGTTAGACGAATATC-3′ 5′-GGAGAGCTCGATATCGCATGTATGGTGATGGGATCTGAC-3′	This study
Confirmation of E. coli MP1 lldD deletion	5′-TGTCAGACGAGGTTGCC-3′ 5′-CAATCTGTGACGCTTGGC-3′	This study
Deletion of E. coli MP1 dld	5′-GCTTCTTCTAGAGGTACCGCATGCGACTGTTTTCACCGCATC-3′ 5′-TCCGTTATTATGTGGTGGCGAAAAAAATATC-3′ 5′-CGCCACCACATAATAACGGATGGCAGAG-3′ 5′-GGAGAGCTCGATATCGCATGCGCTGATGTCTTCAGAAAAC-3′	This study
Confirmation of E. coli MP1 dld deletion	5′-AAGCAGAGACACGCCCG-3′ 5′-AACAGCGAAATCAGCCG-3′	This study
Deletion of E. coli MP1 ykgEFG	5′-GCTTCTTCTAGAGGTACCGCATGGTGAATCATCTTTTCACAAG-3′ 5′-CATATCTCTCACTTCATGCCCATTTATG-3′ 5′-GGCATGAAGTGAGAGATATGTAGTCTGGAC-3′ 5′-GGAGAGCTCGATATCGCATGATGGTCAGGAGATAAGAG-3′	This study
Confirmation of E. coli MP1 ykgEFG deletion	5′-ATCGCTGAGTCAGTAGGC-3′ 5′-ATCAGACAACACCAGGC-3′	This study
Deletion of EcN lldD	5′-GCTTCTTCTAGAGGTACCGCATGGCCGATGATCCGGATTAC-3′ 5′-CGTCTAACCCGCGTTTTTCTCCCTCGAATG-3′ 5′-AGAAAAACGCGGGTTAGACGAATATCTGCTATCCTGC-3′ 5′-GGAGAGCTCGATATCGCATGCGGATACCCCAGCTGGCG-3′	This study
Confirmation of EcN lldD deletion	5′-AGGTGTGCTGCTCAGTCGAC-3′ 5′-GTGAAGTGGTGGAAGAAGCC-3′	This study
qPCR primers for Mus Musculus
Nos2	5′-TTGGGTCTTGTTCACTCCACGG-3′ 5′-CCTCTTTCAGGTCACTTTGGTAGG-3′	[43]
Tnf	5′-AGCCAGGAGGGAGAACAGAAAC-3′ 5′-CCAGTGAGTGAAAGGGACAGAACC-3′	[34]
Ifng	5′-TCAAGTGGCATAGATGTGGAAGAA-3′ 5′-TGGCTCTGCAGGATTTTCATG-3′	[44]
Gapdh	5′-TGTAGACCATGTAGTTGAGGTCA-3′ 5′-AGGTCGGTGTGAACGGATTTG-3′	[44]