Figure 4.

Nanoarray platform sensitivity to biologically relevant multivalent cation concentrations. (A-C) AFM images of origami immobilization post ethanol drying and rehydration on an activated glass substrate through direct incubation in 1, 5, and 40 mM Mg²⁺ buffer, respectively. (D-F) Incubation in 40 mM Mg²⁺ followed by ethanol drying and 2 h rehydration in 1, 5, and 40 mM Mg²⁺ and consequent drying. (G-I) TIRF images and their corresponding FFTs (insets) of Mg²⁺-mediated immobilization on the nanoarray platform with 700 nm pitch at 40 mM Mg²⁺ followed by a 2 h rehydration in 1, 5, and 40 mM Mg²⁺ buffers.