Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Polyclonal anti-Hsf1 | Gross Lab | N A |
| Rabbit Polyclonal anti-Rpb1 | Gross Lab | N A |
| Mouse monoclonal anti-Myc (9E10) | Santa Cruz Biotechnology | Cat #sc-40; RRID:AB_627268 |
| Chemicals, peptides, and recombinant proteins | ||
| TaqI-v2 | New England BioLabs | Cat#R0149 |
| Quick T4 DNA Ligase | New England BioLabs | Cat#M2200L |
| 1,6-Hexanediol | Sigma-Aldrich | Cat#240117 |
| 2,5-Hexanediol | Sigma-Aldrich | Cat#H11904 |
| RNase (DNase-Free) | Sigma Aldrich | Cat#11579681001 |
| Proteinase K | Invitrogen | Cat#25530049 |
| Power SYBR Green PCR Master Mix | ThermoFisher Scientific | Cat#4367660 |
| Protein A Sepharose Beads | GE Life Sciences | Cat#17096303 |
| TRIzol Reagent | Invitrogen | Cat#15596026 |
| DNase I (RNase-free) | New England BioLabs | Cat#M0303 |
| Critical commercial assays | ||
| RNA Clean and Concentrator-25 | Zymo Research | Cat#R1018 |
| Superscript IV first-strand synthesis system | Invitrogen | Cat#18091050 |
| Deposited data | ||
| Hsf1 ChIP-seq | (Pincus et al., 2018) | GEO# GSE117653 |
| Med15 ChIP-seq | (Sarkar et al., 2020) | NCBI Short Read Archive Project# PRJNA657372 |
| Rpb1 ChIP-seq | (Albert et al., 2019) | GEO# GSE125226 |
| NAC-seq | (Pincus et al., 2018) | GEO# GSE117653 |
| Imaging data | This paper | doi: 10.17632/cr972zx459.1 |
| Experimental models: Organisms/strains | ||
|
Saccharomyces cerevisiae W303 and BY4741 strain backgrounds (Table S1) |
This paper | N A |
| Oligonucleotides | ||
| Primers for 3C analysis (Table S2 and S3) | This paper | N A |
| Primers for ChIP analysis (Table S4) | This paper | N A |
| Primers for RT-qPCR (Table S5) | This paper | N A |
| Primers for strain construction (Table S6) | This paper | N A |
| Recombinant DNA | ||
| pFA6a-mCherry-hphMX6 | (Wang et al., 2014) Jian-Qui Wu |
Addgene plasmid # 105156 RRID: Addgene_105156 |
| pSH100 (YCplac33 MET25pro MCP-mCherry | (Hocine et al., 2013) Robert Singer and Daniel Zenklusen |
Addgene plasmid # 45930 RRID: Addgene_45930 |
| pDZ415 (24MS2SL loxP-Kan-loxP) | (Hocine et al., 2013) Robert Singer and Daniel Zenklusen |
Addgene plasmid # 45162 RRID: Addgene_45162 |
| pFA6a-13Myc-His3MX | (Longtine et al., 1998) John Pringle |
Addgene plasmid # 41602 RRID: Addgene_41602 |
| pSH69 | (Hegemann and Heick, 2011) | Euroscarf (P30675) |
| pNH604-HSF1pr-HSF1-GFP | This paper | N A |
| pNH604-HSF1pr-HSF1(147-833)-GFP | This paper | N A |
| Software and algorithms | ||
| Bowtie 2 | (Langmead and Salzberg, 2012) | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| SAMtools | (Li et al., 2009) | http://samtools.sourceforge.net |
| deepTools2 | (Ramirez et al., 2016) | https://deeptools.readthedocs.io/en/develop/ |
| Integrative Genomics Viewer (IGV) browser | (Robinson et al., 2011) | https://software.broadinstitute.org/software/igv/ |
| MACS2 | Zhang et al., 2008 | https://pypi.org/project/MACS2/ |
| TopHat2 | (Kim et al., 2013) | https://ccb.jhu.edu/software/tophat/index.shtml |
| Plotly | (Plotly Technologies Inc., 2015) | N A |
| R | (R Core Team, 2020) | https://www.r-project.org |
| Leica Application Suite-X | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| ImageJ2 | (Rueden et al., 2017) | https://imagej.net/software/imagej2/ |
| YacuDecu | Brian Northan | https://github.com/bnorthan |
| Fiji | (Schindelin et al., 2012) | https://imagej.net/software/fiji/#publication |
| ClearVolume | (Royer et al., 2015) | https://clearvolume.github.io |
| arivis Vision4D | arivis AG, Rostok, Germany | https://imaging.arivis.com/en/imaging-science/imaging-science |
| FindFoci | Herbert et al., 2014 | http://www.sussex.ac.uk/gdsc/intranet/microscopy/UserSupport/AnalysisProtocol/imagej/findfoci |
| MATLAB | MATLAB, 2021. version 9.11.0.1873467 (R2021b), Natick, MA: The MathWorks, Inc. | https://www.mathworks.com/products/matlab.html |
| JACoP | (Bolte and Cordelieres, 2006) | https://imagej.nih.gov/ij/plugins/track/jacop.html |
| Other | ||
| Bioruptor Plus | Diagenode | Cat#B01020001 |
| MatTek glass bottom dish (35mm) | MatTek | Cat#P35G-1.5-14-C |
| Bioptechs Delta TC3 Culture Dish Micro-Observation Temperature Control System | Bioptechs | Model# Delta TC3 |