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. 2022 Nov 25;3(11):1300–1317. doi: 10.1038/s43018-022-00450-6

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© The Author(s) 2022, corrected publication 2022, 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (A) Defining CD4⁺ and CD8⁺ T cells withing the activated T cell cluster (non-responders n = 3 patients, responders n = 9 patients). (B) CITRUS populations identified using differentiating markers (CD3, CD4, CD8, CD14, CD56). (C) Gating strategy for CD45⁺ cells used for clustering analysis. (D) Gating strategy for memory T cells. (E) Quantification of Effector Memory CD4 T cells post treatment by dose of radiation, at time of surgery (18 Gy n = 6 patients, 24 Gy n = 8 patients). Statistical significance was determined using a two-tailed unpaired student’s t-test. The error bars represent the standard error of the mean (± SEM).

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