Extended Data Fig. 3. T-cell independent abscopal effects upon radiation therapy and CD47 blockade in the NJH29 SCLC model.
a. Human SCLC cells (NJH29) were engrafted into both flanks of NSG mice and only right-side tumors were irradiated. b. Immunoblot analysis for ASCL1, NEUROD1, c-MYC. HSP90 is a loading control. N = 1 experiment. c, e. Growth curves of xenografts with the indicated treatments. c. NJH29: N = 1 experiment with n = 5 mice per condition. Irradiated tumors: **p = 0.0022, ***p = 0.0008, non-irradiated tumors: *p = 0.0113, *p = 0.0241. d. CD47 expression by flow cytometry in NJH29 control and CD47 knockouts. e. NJH29: N = 1 experiment with n = 5 mice. NSG mice were engrafted with either two control tumors or two knockout tumors. f. Quantification of tumor volume 8 days after radiation in irradiated and non-irradiated control tumors in (e). N = 1 experiment with n = 5 mice. Irradiated tumors: ***p = 0.0006, ****p < 0.0001, non-irradiated tumors: ***p = 0.0002. Two-tailed t-tests following two-way ANOVA were performed in (c) (irradiated tumors: p = 0.0015, non-irradiated tumors: p = 0.0055). Two-tailed t-tests following one-way ANOVA were performed in (f) (irradiated tumors: p < 0.0001, non-irradiated tumors: p = 0.0001).