Extended Data Fig. 5. Abscopal effects of combination of radiation and CD47 blockade are mediated by macrophages in SCLC.
a Macrophages were depleted with anti-CSF1 antibody treatment as indicated. b. Mouse KP1 SCLC cells were engrafted into both flanks of NSG mice and only right-side tumors were irradiated. Growth curves of KP1 SCLC allografts with the indicated treatments. N = 1 experiment with n = 5 tumors. Irradiated tumors: *p = 0.0398, ****p < 0.0001, non-irradiated tumors: ****p < 0.0001. c. Histological analysis and quantification of macrophages infiltrating irradiated and non-irradiated tumors from Fig. 4c by immunostaining for F4/80. Sections were counterstained with hematoxylin. Each symbol represents one field quantified. Scale bar, 100 µm. N = 1 experiment with n = 5 mice. Irradiated tumors: ****p < 0.0001, **p = 0.0033, non-irradiated tumors: ****p < 0.0001, **p = 0.0045. d. Expression of representative genes for subpopulations of CD45 + hematopoietic cells in SCLC tumors from the scRNA-seq analysis as in Fig. 4d. The % of cells expressing the genes is indicated by the size of the circle. Two-tailed t-tests following one-way ANOVA were performed in (c) (irradiated tumors: p < 0.0001, non-irradiated tumors: p < 0.0001). Two-tailed t-tests following two-way ANOVA were performed in (b) (irradiated tumors: p < 0.0001, non-irradiated tumors: p < 0.0001). Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.