Extended Data Fig. 1. CD47 blockade enhances local antitumor effects following radiation in murine and human SCLC models.
a. Growth curves of KP1 SCLC allografts in NSG mice irradiated with 0, 5, or 10 Gy. N = 1 experiment with n = 5 tumors for each condition. ***p = 0.003, **p = 0.0079. b. In vitro phagocytosis assay performed with mouse bone marrow-derived macrophages (BMDMs) and KP1 mouse SCLC control and Cd47 knockout cells. N = 1 experiment with triplicates of the primary cultures. c–f. Experiments with NCI-H82 SCLC xenografts in NSG mice. c. Growth curves with the indicated treatments. N = 1 experiment with n = 4 mice. ***p = 0.0002, *p = 0.0225. d. Body weight of mice. e. Representative H&E (hematoxylin and eosin) of NCI-H82 tumor sections. f. Histological analysis of macrophage infiltration in SCLC xenografts. Specimens were stained for the macrophage marker, F4/80 (left) and the signal was quantified (right). Scale bar, 100 µm. N = 1 experiment with n = 4 tumors. ****p < 0.0001, *p = 0.0136. g–j. Same as (c–f) for NJH29 SCLC xenografts. N = 1 experiment with n = 4 (Control, RT) or n = 5 (Anti-CD47, RT + Anti-CD47) mice. g. **p = 0.0054, ***p = 0.0009. j. ****p < 0.0001. Two-tailed unpaired t-tests were performed in (b) with primary BMDMs. Two-tailed t-tests following two-way ANOVA were performed in (a) (p < 0.0001), (c) (p = 0.0004) and (g) (p = 0.0007). Two-tailed t-tests following one-way ANOVA were performed in (f) (p < 0.0001) and (j) (p < 0.0001). Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.