Extended Data Fig. 2. Abscopal effects upon radiation therapy and CD47 blockade in the KP1 mouse model of SCLC are independent of T cells.
a. Growth curves of KP1 SCLC allografts with the indicated treatments in irradiated and non-irradiated tumors. N = 1 experiment with n = 5 mice. Independent experiment shown in Fig. 3c. Irradiated tumors: *p = 0.0159, ***p = 0.0001, **p = 0.0079, **p = 0.0023, non-irradiated tumors: ***p = 0.0003, ****p < 0.0001, **p = 0.0079. b. Histological analysis and quantification of T-cell infiltration as in Fig. 3c by immunostaining for CD3. Sections were counterstained with hematoxylin. Each symbol represents one field quantified. Scale bar, 100 µm. N = 1 experiment with n = 5 mice except RT + anti-CD47/Control and RT + Anti-CD47/CD8 depletion (n = 4 mice). c. Populations of macrophages and T cells (antibodies indicated) quantified by flow cytometry in the KP1 and KP3 mouse allograft models in B6129SF1 hosts. N = 1 experiment with n = 4 (KP1) or n = 5 (KP3) mice. *p = 0.0317, *p = 0.0159. d. KP3 SCLC cells were engrafted into both flanks of B6129SF1 immunocompetent syngeneic mice and only right-side tumors were irradiated. e. Growth curves of KP3 allografts with the indicated treatments in irradiated and non-irradiated control tumors. N = 1 experiment with n = 7 (Anti-CD47) or n = 8 (Control), or n = 9 (RT and RT + Anti-CD47) mice (2 tumors per mouse). 5/9 mice had complete remission at both sides in the combination treatment arm. ****p < 0.0001. Two-tailed t-tests were performed in (c). Two-tailed t-tests following one-way ANOVA were performed in (b) (irradiated tumors: p = 0.0054, non-irradiated tumors: p = 0.30). Two-tailed t-tests following two-way ANOVA were performed in (a) (irradiated tumors: p < 0.0001, non-irradiated tumors: p < 0.0001) and (e) (irradiated tumors: p < 0.0001, non-irradiated tumors: p < 0.0001). Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.