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. 2022 Nov 26;5:1296. doi: 10.1038/s42003-022-04258-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic of optimized NcCas9 and its sgRNA architecture. b Genome editing of NcCas9 with N4GYAT, N4ATAT and N4GTTT PAMs in 48 endogenous loci. c Top: Schematic of the nNc-CBE. Bottom: Efficient C-to-T conversions in 12 endogenous loci by four nNc-CBE. d Top: Schematic of the nNc-ABE, TadA 8e was used. Bottom: Efficient A-to-G conversions in 12 endogenous loci by nNc-ABE. The highest C-to-T/A-to-G editing frequency within the editing window represents the base editing frequency at each site. Error bars indicate s.e.m. (n = 3).