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. 2022 Nov 26;5:1296. doi: 10.1038/s42003-022-04258-z

Fig. 4. NcCas9 is inhibited by Acr proteins in human cells.

Fig. 4

a Schematic of Acr proteins is used to inhibit the activities of NcCas9 and its base editors. b Genome editing of NcCas9 in the presence or absence of the 14 previously described Acr families. Plasmids expressing NcCas9, sgRNA targeting site5, and each Acr (1:1:1) were co-transfected into HEK293T cells. c Base editing of nNc-CBE in the presence or absence of six Acrs at the site1. d Base editing of nNc-ABE in the presence or absence of six Acrs at site1. Error bars indicate the s.e.m. (n = 3).