TABLE 2.
Reduction of the bactericidal activity against Escherichia coli ATCC 29648 after adsorption of the peptides with live Brucella or Salmonella strainsa
| Peptide | % Reduction in bactericidal activity
|
|||
|---|---|---|---|---|
| B. abortus S19 | B. abortus 45/20 | B. ovis REO 198 | S. montevideo | |
| Bactenecin 5 | 3 ± 0.25 | 34 ± 4.00 | 34 ± 1.00 | 16 ± 1.10 |
| Bactenecin 7 | 5 ± 0.25 | 20 ± 2.50 | 40 ± 5.00 | 55 ± 2.00 |
| Poly-l-lysine | 3 ± 0.25 | 20 ± 2.50 | 20 ± 2.50 | 30 ± 2.50 |
| Poly-l-ornithine | 12 ± 1.00 | 20 ± 2.50 | 30 ± 2.00 | 40 ± 3.00 |
| Melittin | 17 ± 1.40 | 41 ± 4.00 | 53 ± 3.10 | 41 ± 2.15 |
| Lactoferricin B | 5 ± 1.25 | 25 ± 2.00 | 50 ± 2.50 | 60 ± 3.00 |
| Polymyxin B | 27 ± 1.5 | 47 ± 1.50 | 60 ± 3.50 | 71 ± 3.50 |
a
Ten micrograms of the peptide to be tested was mixed with 1010 bacterial cells in a 0.1-ml volume. The mixture was centrifuged and 15 μl of the supernatant was dispensed into wells punched in peptone-glucose-agar plates previously inoculated with E. coli ATCC 29648 as target cells, as described previously (9).