Skip to main content
. 2022 Nov 26;13(11):1002. doi: 10.1038/s41419-022-05449-6

Fig. 3. PFKP Y64 phosphorylation induces VEGF expression through HIF-1 α expression and β-catenin Ser552 phosphorylation in response to EGFR activation.

Fig. 3

WB and qRT-PCR were performed with indicated primers and antibodies, respectively (A–E). A LN229/EGFRvIII cells with or without PFKP depletion and with or without reconstituted expression of WT Flag-rPFKP or Flag-rPFKP Y64F mutant were transfected with or without Flag-HIF-1α and HA-myr-AKT. B Serum starved LN229 cells were pretreated with DMSO or HIF inhibitor (10 μM) for 1 h and then treated with EGF (100 ng/mL) for 12 h. C LN229 cells were transfected with a control vector or CA β-catenin. D LN229/EGFRvIII cells with stable expression of β-catenin shRNA or a control shRNA were reconstituted with or without WT rβ-catenin or rβ-catenin S552A mutant. E PFKP-depleted LN229/EGFRvIII cells with or without reconstituted expression of WT Flag-rPFKP or Flag-rPFKP Y64F mutant were transfected with or without CA β-catenin and Flag- HIF-1α. Data are presented as mean ± standard deviation of three independent experiments (A–E). **P < 0.01; ***P < 0.001, based on the Student’s t-test or one-way ANOVA with Tukey’s post hoc test.