Fig. 7. interactions between the FoxH1 FH domain and a native Gsc promoter nucleosome.
a Gsc boundaries (dashed lines) were selected based on the MNase seq information described in GSM2842982 (mouse). The boundaries are more similar in muscle, heart, brain and liver than in kidney datasets, with differences perhaps indicating NCP dynamics in the different conditions. In this work, we used the Gsc sequence corresponding to the (a) site. The Gsc human NCP sequence (in light yellow) contains the FoxH1 site (highlighted in orange). Both human and mouse sequences are highly conserved. b Titration of the native Gsc167 with FoxH1 FH domain. The Gsc167 sequence and the FoxH1 motif are indicated. DNA ladder is included as a reference for the linear DNA. c Comparison of the binding affinities of FoxH1 and FoxA2 to the Gsc sequence (NCP and linear DNA). Bars represent fitted KD values and error bars represent standard error of the fit (n = 5). Source Data are provided.